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Anions in Beer: Fluoride, chloride, phosphate and sulfate

Beer - quality control by anion monitoring

The majority of anions found in beer are a consequence of the water (contains chloride, phosphate, sulfate, nitrate, fluoride) used during the brewing process.

Monitoring the anion profile is an important quality control step in the brewing industry because anions can affect the flavor of the finished product.

If the level of chloride is above 250 mg/L, then the sweetness of the beer is enhanced but it can hinder yeast flocculation, so it needs to be carefully observed. An excess of sulfate has been found to give a sharp, dry edge to well hopped beers and so the level present should be minimized as much as possible bearing in mind that sulfate occurs naturally in water. Phosphate is present in the malt and buffers the mash to a slightly acidic pH. Quantifying the level of nitrate is important as excessive amounts can hamper the fermentation process after conversion to nitrite.

The finished beer product and intermediates used throughout (such as the brewing water) can easily be determined using ion chromatography to ensure manufacturing authenticity.


Analysis of beer by ion chromatography

Metrohm’s well known MIC-2 Advanced modular anion system with chemical suppression is used for the analysis of beer. In the method described herein, light beer wort is being analyzed for the determination of fluoride, chloride, phosphate and sulfate contents.

Simple sample preparation steps and the need of only small amounts of samples are the major advantages of ion chromatography as an analytical technique: dilution is the only sample preparing step required. Dilution and moreover the whole beer analysis can easily be automated using the 838 Advanced IC Dilution Sample Processor.

Detection is performed with the high-performance 819 IC Detector (conductivity detector) after chemical suppression with Metrohm’s built-in Suppressor Module (MSM). The MSM with its independent three micro-bed packed suppressor is very important for reproducibility. A fresh suppressor channel is used each time for analysis ensuring that the cation exchanger is operating at full capacity. The remaining two channels are either regenerated or rinsed.


Product information

Modular ion chromatography system with Metrohm Suppressor Module (MSM, with 10 years warranty) MIC-2 Adv
Metrosep A Supp 5 - 150 separation column 6.1006.520

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838 Advanced IC Dilution Sample Processor 2.838.0120

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Tips

  • Automation and Sample preparation: The 838 Advanced IC Dilution Sample Processor can be used for sample dilution and fully automated beer analysis as well.
  • Validation: For the Validation of Metrohm ion chromatography systems using Standard Operating Procedures see our Application Bulletin 277 (Info Center).
 
   
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