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Chemical Analysis of Honey

Honey - Nature's Sweetener

Honey is widely used in food products, cosmetics and ayurvedic medicines. It contains a variety of sugars (e.g. fructose, glucose, maltose, sucrose).

Honey is composed mainly of different sugars, traces of pollen and water. In smaller amounts, acids, proteins and minerals are also present. The typical composition of honey is: fructose 38.38% (range: 30.90 – 44.25%); glucose: 30.30% (range: 22.90 – 40.75%); water 17.2% (rage: 13.4 – 22.9%), maltose: 7.31% ( 4 – 8%) and sucrose: 1.31% (range 0.25 – 7.57%).

Fructose is about two and a half times as sweet as glucose. From the numbers above it can easily be seen that fructose and glucose are present in natural honey in a ratio of 1.2:1. As honey is widely used in food products, cosmetics and ayurvedic medicines, the perspective of additional gains by adulterating honey seems to be tempting.


Titration offers a fast way to determine the sum of all reducible sugars (e.g. glucose, fructose, maltose and lactose). Electrochemically inert sugars (non-reducible sugars as sucrose) can’t be analyzed by normal titration.

If you need to distinguish between different kinds of sugars, ion chromatography is the method of choice as you gain more detailed information about your sample than by titration.


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